An Increase of the Total Solidification Temperature in Salt Media by Dextran Protection
نویسندگان
چکیده
An investigation was performed on the possibilities for an alteration of the total solidification temperature when preserving concentrated salt media (BME, MEM, 199, HAM), used for the maintenance of animal viruses and cell cultures. It had been established that by the use of dextran 40000, this temperature could be increased with about 20C. A protection in 3.6 % concentration was recommended and the freezing to minus 40C might be performed directly in the refrigerator for storage. The temperature should not be raised over minus 32C during the stockage.
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